Effects of truffle inoculation on a nursery culture substrate environment and seedling of Carya illinoinensis

We inoculated Tuber aestivum and sinoaestivum on Carya illinoinensis to explore the effects of inoculation host plant growth, enzyme activities, physicochemical properties rhizosphere soil, denitrifying bacterial community in rhizosphere, distribution mating type genes rhizosphere. found that spp. increased height stem circumference was greater two months after inoculation. Six inoculation, peroxidase activity seedlings with T. higher than control. At four six superoxidase dismutase activities were those sinoaestivum. nitrate nitrogen content lowest control highest treatment. Among nirS-type bacteria community, relative abundances Proteobacteria high. did not affect diversity bacteria. The MAT1-1-1 MAT1-2-1 detected C. aestivum, dominated over MAT1-2-1.